Introduction Micro propagation of plants, an avant-garde application of biotechnology in various agrobiological branches, ensures the obtaining of planting material with a high biological value that meets important criteria: genetic uniformity, quality of the planting material and price, being also a method of getting rid of pathogenic agents of the seedlings. This method aims at the development and optimization of in vitro propagation technologies for new species of fruit and ornamental shrubs: Licium barbarum L.,Lonicera kamtschatica (Sevast.)Pojark, Amelanchier canadiensis (L.) Medic., Actitinidia arguta (Siebold. & Zucc.) Planch. Ex Miq., Actinidia colomicta (Maxim. & Rupr.) Maxim., as well as of some frost-resistant varieties of Rubus and Ribes species. Material and metods The experimental works for the initiation of cultivars of fruit shrubs involve the identifi cation and description of the species and varieties for which in vitro propagation technologies will be developed. The choice of the optimal explants with a high rate of propagation, determination for the species, variety, of the optimal period for the initiation of the cultivation in vitro of the explant type, of the sterilization and disinfection formula, of the growing medium and hormonal balance are performed in order to obtain optimal propagation rates. The ex vitro acclimatization of vitro cultures is a special process including the hydroculture by fl otation, which is a new element in the vitro techniques of the laboratory. Rezults and discussions The shrubs initiation was experimented by testing the explants of the apical and lateral meristem and 8 variants of medium. In order to initiate in the culture in vitro, as explants, apical and lateral meristems are recommended, shoot fragments with multiple nodes and whole leaves and fragments of leaf lamina depending on the species, variety or cultivar. The regeneration and growth of axillary shoots is peculiar to each taxon, there are generated one after another from 3 to 35 shoots, and, rooted plantlets are obtained after 2-3-4 months of cultivation in vitro. In the initiation phase, as well as in the reproduction phase, adventitious roots regenerate on the shoots. Among the nutritional variants of medium which were tested, there are two basic ones with some modifi cations: MS-100%-Murashige&Skoog 1962 and Woody Plant Medium according to Lloud and McCown, growth regulators are 2 izopentenyladenine and zeatin. Both proved to be effective, for Wacccinium and Amelanchier, modifi ed WPM with 5mg/l 2-Ip or 1-2 mg/l zeatin stood out and provided a good proliferation rate and relatively well developed shoots, the micro cloning or sprouting was produced in vitro with 10 mg/l 2-Ip generating tens of thin shoots per explant. The varieties and cultivars of the genus Ribes, Rubus, Tayberry, Licium, Schisandra, Actinidia, Lonicera proliferated on basal media MS- 100% supplemented with growth regulators BAP and AIA in concentrations from 0.3 to 0.7 mg/l. Conclusions The species, varieties and cultivars of fruit shrubs can be successfully introduced and cultivated in vitro in basal media MS supplemented with growth regulators, were obtained results at the taxa of the genera Ribes, Rubus, Tayberry, Licium, Schisandra, Actinidia, Lonicera, using Wacccinium and Amelanchier on Woody Plant Medium according to Lloud and McCown, the growth regulators being 2 izopentenyladenine and zeatin.