The versatility of 2,6-diacetylpyridine hydrazones in generating varied molecular architectures requires combination of various analytic techniques, the NMR methodology being one of the most powerful for characterization of the self-assembled complexes and investigation of their dynamic behaviour in solution [1]. 2,6–Diacetylpyridine bis(nicotinoylhydrazone) (H2L) was first reported by P. Mazza et al. as a part of their research devoted to investigation of the chemical, structural, and biological properties of some new organotin acylhydrazone derivatives [2]. 119Sn NMR data for characterization of the organotin compounds were presented, while H2L was only identified by IR and mass spectrometry data.  The current report presents some complementary data on H2L. For the first time its spectral 1H and 13C NMR individual characteristics that were obtained in DMSO-d6 solution are presented. The relevant NMR spectroscopic data for structure elucidation were furnished by 1D (1H, 13C, DEPT- 90o) and 2D (1H/13C HSQC and 1H/13C HMBC) HETCOR experiments. NMR spectra were recorded on a Bruker AVANCE 400 spectrometer equipped with a 5-mm broadband reverse probe with field z gradient, operating at 400.13 and 100.61 MHz for 1H and 13C nuclei respectively. DMSO-d6 (isotopic enrichment 99.95%) was used as solvent, containing tetramethylsilane (TMS) as an internal standard. Chemical shifts (δ) are reported in parts per million (ppm) and are referenced to the residual non-deuterated solvent peak (2.50 ppm for 1H and 39.50 ppm for 13C). Coupling constants (J) are reported in Hertz. Multiplicity of signals- s: singlet, br. s: broad singlet, dd: doublet of doublets, ddd: doublet of doublet of doublets, dt: doublet of triplets, m: multiplet.