At present period of agri-industrial production in Ukraine, the following technologies of industrial vermi-cultivation in Ukraine were studied: bio-humus production on various organic waste. Since 2005 in Ukraine, the vermi-farms re-emerged numbering more than 50 today. It is known, that the earthworms play an important role in the processes of decomposition of organic matter and creation of soil structure. They are used for processing of various waste. The process of vermi-cultivation depends mainly on the substrate quality and worms maintenance. The influence of the composition of organic raw material on the fermentation process and the amount of worm mass in the production of biohumus 0077as studied. It was found that the most effective is the composition of organic raw materials with the addition of 29% waste of mushroom blocks of champignons at which the number of worms increases 1.3 times, based on 100% - mushroom blocks of champignons - 1.5 times, based on manure of cows - in 1, 6 times.