For the initiation of in vitro cultures of goji, shoot apex pieces, inoculated in culture medium Murashige-Skoog (MS), supplemented with hormones of different concentrations (α-NAA – alpha-naphthalene acetic acid and BAP – benzylaminopurine) were used (tab. 1). When higher doses of NAA and BAP were used, there was a proliferation of shoots and an increase in their length in the Ningxia N1 variety. The disinfection of the plant material was performed according to the method optimized in the laboratory. The obtained results denote that the growth medium used for the propagation of shoots derived from meristems can significantly influence the process of in vitro rhizogenesis. The experiments aimed at determining the rooting capacity of plantlets in vitro were organized depending on the culture medium. Thus, irrespective of the combination and concentration of growth hormones, it had a positive influence on the rooting capacity of the shoots, the number and length of the roots formed. In the case of the plantlets that regenerated in V4 and V6 medium, for which the concentration of IAA was increased to 2.0 mg/l and IBA – to 1.0 mg/l, the rooting rate was 73% and 93%, respectively.