Microorganisms that promote plant growth provide support to the plant in many factors, including nutrition, increased tolerance to stress factors, and stimulating plant development. Although these bacteria exist in nature, effective individuals need to be evaluated to serve farmers. Therefore, a series of survey studies required from several origins to obtain the most effective isolates. At this stage, it has become important to understand which origins of bacteria may be more likely to provide effective PGPR. This report compiles the trial findings conducted by our work group to provide recommendations to researchers interested in isolating PGPR. The results demonstrated that isolating bacteria from diverse habitats other than conventional agricultural sites causes considerable favorable benefits. Recent studies have shown that bacteria isolated from organic farming areas may be more effective in the uptake of micro and macronutrients. This finding may also indicate a richer biodiversity of soils in organic farming areas.