Introduction: Kinetotherapy is indicated as the non-pharmacological treatment method for knee osteoarthritis (OA) by the EULAR and OARSI recommendations. The importance of manual therapy as specific form of kinetotherapy has not been studied in small group. Objective: To evaluate the effectiveness of conventional conservative kynetotherapy combined with manual physical therapy in patients with knee OA. Methods: According to American College of Rheumatology (ACR) criteria, knee OA patients were included in a randomized, controlled, open-label trial and treated in University Rehabilitation Center, in the Republic of Moldova. Participants received both a conventional kinethoterapy and manual therapy, over 10 days. For functional knee evaluation, we used the evaluation of joint mobility with goniometer and muscle strength from 0 to 5 points. The pain was assessed by Visual Analogue Scale (0 to 100mm). This study was conducted according to the principles of the Declaration of Helsinki (1996) and good clinical practice. Results: There were 28 patients integrated in the study including 20 females and 8 males, mean age 64.19±6.9 years (range 42 to 72 years). Disease duration was 8,7±6.4 years (range 1-22). The pain level was78,5±4,7mm. The patients with knee OA have improved the knee flexion after kinetotherapy with 18±4.0 degree (p<0.05), and knee internal and external rotation with 7±2,9 and 6 ±1,7 degree respectively (p<0.05), but there were no changes in the articular extension. We also established the significant decrease of pain level (p<0.05). Conclusion: Results suggest that manual therapy treatment is beneficial in reducing knee OA pain and improvement of joint mobility. Further studies should be conducted to clarify the role of different treatment components and non-specific effects.