Plant breeding is the remarkable applied discipline of plant genetics with the aim of crea-ting new genotypes with high-performing characters and properties, both in terms of production level, as well as yield quality and resistance to extreme environmental conditions [1, 2]. For the creation of new forms of tomato with increased resistance to extreme temperatures and with high productivity under water deficiency conditions, different intra- and interspecific hybridi-zation methods were used. Interspecific hybridization is a method that can induce much higher variability compared to intraspecific hybridization. This method is used to create an initial ma-terial for tomato breeding by transferring from wild species such as L. chilense, L. peruvianum and L. hirsutum to the cultivars some valuable characters, such as resistance to stress factors and useful dry compound content in fruits. Although worldwide there is a large number of hy-brids, varieties, lines, and in our country the tomato assortment is vast including genotypes for field cultivation of determinate and semi-determinate growth type intended for industrialization or fresh consumption, the breeding of new varieties always remains up-to-date.  Purpose: research and assessment of quantitative traits in F2 hybrid populations based on characters of interest for breeding.