Steroidal glycosides (SG) - secondary metabolites of higher plants, is a common group of biologically active compounds and its study is the subject of numerous research works in the field of chemical and biological knowledge. The range of their physiological action and the level of biological activity depend on their chemical structure, the concentration used and the method of application of these substances. Their antioxidant properties, hypocholesterolemic, growth regulator, fungicide action make this class of biologically active compounds attractive for detailed study because, along with low toxicity, the availability of sources of them can be recommended for practical use in crop production and medicine. To identify additional sources of biologically active compounds of glycoside nature, which posses a high level of activity, we examined a number of representatives from fam. Scrophulariaceae of wild flora Moldova, pharmaceutical properties of which are used in traditional and folk medicine. These include some types of Verbascum, Linaria, Digitalis. From total extracts of each plant by chromatographic methods individual compounds were obtained, the chemical structure of which defined by means of spectral analysis methods. Steroidal, phenol, iridoid glycosides were identified among the isolated compounds, some of which were obtained for the first time. As well as for a summary of glycoside fractions and individual compounds was determined the spectrum of biological activity. Thus, the glycosides from Linaria vulgaris Mill. for pre-soaking of seeds of sweet pepper in their solutions, increase the stability of this culture to cold stress at 40% regardless of the grade. Glycosides from Verbascum phlomoides L. increase the seed production of medicinal plants up to 70%. These compounds induce the resistance of crops to abiotic and biotic stresses. All this, along with the availability of raw materials, can be recommended for detailed studies to extensive use in agricultural practices.