The development in the field of high-energy chemistry, in particular, with the use of low-temperature plasma electrolysis (LTPE), for the study of liquid-phase processes is important in the way of creating completely new technologies that are distinguished by high efficiency and the possibility of their wide application in industry. The uniqueness of the LTPE method lies in the fact that one electrode is located in the liquid phase, and the other is placed at a certain distance from the surface of the liquid, which makes it possible to induce nonequilibrium redox processes in the treated liquid, the implementation of which is impossible when using traditional electrolysis or the effect on plasma liquid of arc, crown, Townsend, or barrier discharges. Previously, we studied the purification of waters of various origins from organic substances and radioactive elements and showed its high efficiency. In this study, we have studied the effect of low-temperature plasma electrolysis on solutions containing various types of bacteria and viruses, both when processing certain volumes of liquid media and during electrolysis in a reactor with a dynamic liquid film. The influence of LTPE on solutions containing pathogenic, conditionally pathogenic and indicator bacteria is considered. The high efficiency of the effect of low-temperature plasma electrolysis in the disinfection of bacterially contaminated drinking and waste water has been shown. The influence of LTPE on the death of viruses in water was investigated. Established high virulence against poliomyelitis virus and coliphages. An experimental study of the effect of various modes of disinfection of drinking water and wastewater on the survival of bacteria: Salmonella, Clostridia, bacteria of the E. coli group, lactose-positive E.coli was carried out according to the following bacteriological indicators: total microbial count, lactose-positive E.coli, clostridia, enterococci and salmonella. It is shown that carrying out electrolysis in an electrolysis cell with a dynamic liquid film of solutions containing various microorganisms makes it possible to efficiently process solutions at concentrations of microorganisms significantly exceeding the maximum permissible norms.