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SM ISO690:2012 MAZUR, Ecaterina, UNCU, Livia. Determination of potassium orotate in mechanical mixture with spironolactone, potassium aspartate and magnesium by UV-Vis. In: Cercetarea în biomedicină și sănătate: calitate, excelență și performanță, Ed. 1, 20-22 octombrie 2021, Chişinău. Chișinău, Republica Moldova: 2021, p. 426. ISBN 978-9975-82-223-7 (PDF).. |
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Cercetarea în biomedicină și sănătate: calitate, excelență și performanță 2021 | ||||||
Conferința "Cercetarea în biomedicină și sănătate: calitate, excelență și performanță" 1, Chişinău, Moldova, 20-22 octombrie 2021 | ||||||
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Pag. 426-426 | ||||||
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Background. The UV-Vis spectrophotometry is used successfully in the analysis of fixed-dose combination (FDC). The determination of the active substances with the different chemical structures leads to difficulties in the development of the spectrophotometric method by selection of solvents, wavelengths. Objective of the study. The development of the spectrophotometric method for determination of potassium orotate (OP) in mechanical mixture with spironolactone (SP), potassium aspartate (AP) and magnesium (AM). Material and Methods. The spectrophotometric measurements were carried out using an UV-Visible spectrophotometer Agilent 8453 with a 1.00-cm quartz cell, standard and samples of the analyzed substances (Sigma-Aldrich, Molekula), 0.1 M NaOH solution. Results. The absorbance of the solutions of the individual substances in 0.1 M NaOH solution was determined: OP showed a maximum at 286 nm and SP - two absorption maxima at 248 and 292 nm. AP and AM showed no absorbance in the UV region due to the lack of chromophore groups. It was found that OP and SP showed maxima in the tetracomponent mechanical mixture at the same wavelength as the individual substances. It was concluded that there is a lack of mutual interference in the absorption process. Following the evaluation of the obtained results, the content of OP in the mechanical mixture was determined 1: 1: 101.3% (RSD 0.23%). SP showed a reduced content (61.4%, RSD 0.27%) due to a low solubility. Conclusion. The developed UV-Vis spectrophotometric method can be applied for the qualitative and quantitative analysis of OP in combination with SP, AP and AM. |
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Cuvinte-cheie UV-vis spectrophotometry, fixed-dose combination drugs, potassium orotate, spectrofotometrie UV-VIS, medicament combinat, orotat de potasiu |
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