Identification of species belonging of pollen material in samples using DNA barcoding is a new promising direction in palynology. Palynological information is in demand in a broad range of areas: monitoring of biological diversity, allergological research, detecting the cases of smuggling and adulteration of food and medicinal raw materials, as well as for obtaining additional information during the investigation of criminal cases. The purpose of our work is to adapt a method of species DNA identification of pollen for solving problems of a forensic nature. A prerequisite for the successful species identification of pollen material is the selection of markers – the so-called DNA barcodes from the currently proposed phylogenetic plant markers. We selected two most common markers – ITS2 and rbcL, which were sequenced in 5 species of woody plants growing on the territory of the Republic of Belarus: the common aspen (Populus trеmula), the ash-leaved maple (Аcer negundo), the common hazel (Corylus avellana),the black alder (Alnus glutinosa), and the goat willow (Salix capreal). ITS2 is a region of the sequence of the internal transcribed spacer of ribosomal genes, which is locatedin nucleus chromosomes in the form of a large number of copies, and therefore, is present in the pollen genome. The rbcL marker is a fragment of the chloroplast gene ribulosebisphosphate carboxylase, which is identified in the pollen grain in species with the paternal inheritance of plastids or may be found in chloroplast precursors – in leukoplasts. In order to isolate DNA from pollen, we used the commercial kit ReliaPrep™ FFPE gDNAMiniprep System (Promega, USA) with a preliminary stage of pollen incubation in mineral oil at 80 °C. The sequences obtained by us were compared with the fragments of the corresponding barcodes presented in the NCBI. According to the results obtained, the pollen of the common hazel and the black alder is equally accurately determined to the species by both markers with a probability of 100% and 99.71% respectively for ITS2and for rbcL– 100% and 99.79% respectively. The ash-leaved maple was identified using ITS2, the similarity with the barcodes presented in the NCBI was 99.67%. In cases of the common aspen and the goat willow, pollen identification using a two-locus combination of ITS2 and rbcL markers was insufficient, and we plan to use additional barcodes, e.g. psbAtrnH and matK. Thus, with a view of identifying the species of wild woody plants by pollen material, it is possible to use a two-four-locus combination of DNA markers: ITS2 and rbcL as the main ones and several more chloroplast barcodes as additional ones.