Article is devoted to the researchers in biotehnology areoc. Are presented the results of investigation of employees achieved over abaut 25 years sinca the creation of this subdivision of Botanical Garden. At the gegenning, works were devoted te recovery of some plants collection on open and protected lands since 1996, along with acquirement development of methods, the level of researches vas thorough according new work program, was studied morphogenetic potential, organogenous and calusogenous processes, ginogenous and androgenesis. Elaboration of technologies for a number of new species (20) with an economic interest for  Republic of Moldova. Biotechnology is defi ned broadly as technologies that involve the use of living organisms, or products from living organisms, as way to benefi t humans. The study, regarded in biotechnology light, of the problem concerning culture tissue and organs in higher plants, out of organism, on the artifi cial nutritive mediums and, in aseptically controlled conditions, opens new possibilities of fundamental and applied investigations.