For the in vitro propagation of Stevia rebaudiana Bertoni for the obtention planting material, Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/l BAP  was used for the multiplication phase. In order to reduce costs in the multiplicaton phase, agar was successfully substituted with wheat starch or liquid medium was used together with paper as mechanical support. For in vitro rooting, hormone-free MS medium was used and a maximum number of microcuttings were inoculated/vessel in order to establish the rooting percentage while using, with maximum effi ciency, the space in the culture vessels. The ex vitro acclimation of rooted plantlets was carried out by using hydroculture. Cuttings were excised from the acclimated plants, which in turn were rooted in fl oat hydroculture.