During the last years, the biologically active substances and products together with the
biological processes have become the main research targets, therefore there is need of analytical
methods and techniques that are not so “classical” and that could solve the analytical issues
occurring in solution. The use of modern analytical procedures makes possible the composition
analysis, the determination of the concentration domain for each component, the structural
characterisation of the components that could finally lead to the clarification of the action
mechanism, its transformation paths at the level of investigated bio-system.
The bioanalysis concept, came to light recently in the current language of the analytical
chemists and biochemists, has a pronounced interdisciplinary character, but it has “grown”
significantly fast thus turning into a scientific domain having a special dynamic [1]. Bioanalysis
domain started from substances such as drugs and their metabolites achieving the quantification
of biological products with complex molecules having biopharmaceutical interest, such as
proteins, peptides or DNA. Along with the proliferation of sophisticated techniques that link
advanced separations with mass spectrometry or NMR as detection systems, and the involvement
of automation and robotics, bioanalysis faces the nowadays challenges in current analysis of
xenobiotics from biological matrix more quickly and with a higher degree of confidence,
discover new biomarkers that underlie the development of diagnostic kits to develop
personalized medicine. Bioanalysis includes different fields of application as well as samples
that differ as composition (biological fluids, cells and tissues, drugs and metabolites, food, etc.).
It is obviously that all these application domains provide samples having a highly complex
matrix that could drastically influence the quality of determinations. The use of bioanalytical
methods should take into account the fact that in many cases, the normal concentrations are near
the detection limits of the used instrumental techniques, thus promoting the use of fast
techniques that require a low pre-treatment of the sample.
Bioanalysis represents a research domain for which the future has many exciting
opportunities such as continuous improvement of efficacy, data processing, reliable results,
reducing analysis costs and environmental impact. Many future scientific efforts are directed
towards a sensitive, selective and precise quantification of drug substances, as well as of
endogenous biological substances to provide complete information that is necessary in areas
such as: pharmacokinetics, toxicokinetics, bioequivalence, metabolomics, etc. Bioanalysis is also
involved in the detection of substances that are used for illicit purposes, forensic investigation
and concerns regarding environmental quality monitoring.
References:
[1] Tehnici experimentale in bioanaliza. Vol. I-VIII, Editura Printech: Bucuresti.