Pseudomonas aeruginosa is the most important of all pseudomonads from the perspective of clinical laboratory isolation frequency, variety, morbidity and mortality of caused infections. (Buiuc, 2009).  Study group: 20 strains of Pseudomonas aeruginosa isolated from animals that were brought to the Medical Clinic of the FMV of Iaşi for investigation and treatment.  The performances of isolation media in stimulating pigment production vary. The best results were obtained on nutrient agar base medium for Pseudomonas (Merck, Oxoid).  Biochemical confirmation of the strains in this study was done by using the API 20 NE and Rapid NF Plus tests, using both tests to highlight the advantages of Rapid NF Plus tests.  For a good and accurate evidentiation of Pseudomonas aeruginosa strains we recommend isolating the strains on Pseudomonas agar base using cetrimide (Merck) as selective supplement: the pigment appears highly pronounced compared to other growth media, as for biochemical confirmation we recommend the RapID NF Plus test since it provides the main advantage of reducing the identification time to 4 h.