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![]() TATAROV, Pavel, IVANOVA, Raisa, BAERLE, Alexei, SANDU, Iuliana, BRINDZA, Ján. Walnut kernels pellicle (Juglans regia l.) – a good source of gallic, ellagic acids, catechin and their derivatives for functional foods. In: Agrobiodiversity for improving the nutrition, health: , quality of life and spiritual human development, Ed. 5, 3 noiembrie 2021, Nitra. Nitra, Slovacia: Slovak University of Agriculture in Nitra, 2021, Ediția 5, p. 123. ISBN 978-80-552-2401-5. 10.15414/2021.9788055224015 |
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Agrobiodiversity for improving the nutrition, health Ediția 5, 2021 |
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Conferința "Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and Spiritual Human Development" 5, Nitra, Slovacia, 3 noiembrie 2021 | |||||||
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Pag. 123-123 | |||||||
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The pellicles, which cover the walnut kernels, protect the lipids from the kernel’s |
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Cuvinte-cheie antioxidant activity, functional foods, gradient elution, HPLC, kernel’s pellicle, polyphenols |
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Cerif XML Export
<?xml version='1.0' encoding='utf-8'?> <CERIF xmlns='urn:xmlns:org:eurocris:cerif-1.5-1' xsi:schemaLocation='urn:xmlns:org:eurocris:cerif-1.5-1 http://www.eurocris.org/Uploads/Web%20pages/CERIF-1.5/CERIF_1.5_1.xsd' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' release='1.5' date='2012-10-07' sourceDatabase='Output Profile'> <cfResPubl> <cfResPublId>ibn-ResPubl-141881</cfResPublId> <cfResPublDate>2021</cfResPublDate> <cfVol>Ediția 5</cfVol> <cfStartPage>123</cfStartPage> <cfISBN>978-80-552-2401-5</cfISBN> <cfURI>https://ibn.idsi.md/ro/vizualizare_articol/141881</cfURI> <cfTitle cfLangCode='EN' cfTrans='o'>Walnut kernels pellicle (Juglans regia l.) – a good source of gallic, ellagic acids, catechin and their derivatives for functional foods</cfTitle> <cfKeyw cfLangCode='EN' cfTrans='o'>antioxidant activity; functional foods; gradient elution; HPLC; kernel’s pellicle; polyphenols</cfKeyw> <cfAbstr cfLangCode='EN' cfTrans='o'><p>The pellicles, which cover the walnut kernels, protect the lipids from the kernel’s<br />endosperm against the oxidative degradation. In previous studies, we showed that for the<br />formation of the bad brown colour and bitter taste of walnut kernels, the oxidized polyphenols<br />(naphthoquinones) are responsible. It is necessary to mention that the ratio between the<br />average content of total polyphenols in pellicles and in kernels can reach 12.7, and this<br />excellently correlated with mass fraction of pellicle in the kernels (10–11 %). The majority<br />(more than 90 %) of phenolic compounds are concentrated in the pellicle.<br />The purpose of this research was to found the optimal extraction’s conditions of phenolic<br />compounds from kernel pellicles and to evaluate the antioxidant capacity of these. The yield of<br />extractive components was determined in function of the concentration of food-grade ethanol<br />as solvent, temperature of extraction and previous defatting procedure. Defatting was realized<br />by hydraulic pressing at 20MPa. Results showed that the maximum amounts of phenolic<br />compounds were extracted with 50 % aqueous ethanol at 22–24 °C from the pellicles, obtained<br />from native not-defatted kernels. The total polyphenol content in extract from defatted kernels<br />was of 1.2–1.4 times higher than from the native kernels. But we observed, that the<br />contamination of kernel’s pellicle with the lipids, extracted from endosperm during pressing,<br />make much more difficult extraction of phenolic compounds from the pellicle with aqueous<br />ethanol solutions. The optimization of extraction conditions gives the opportunity to obtain the<br />extracts with total phenolic content increased by 1.5–2.0 times. Due to a rich phenolic content,<br />in dry residue consisted approximately 55–60 %, these extracts exhibited pronounced<br />biological activity. HPLC analysis of polyphenols was performed at "Shimadzu LC-2030C 3DPlus"<br />with PDA detector, by gradient elution with water: acetic acid (polar phase A) and<br />acetonitrile: acetic acid (non-polar phase B), using reversed-phased C18 column "Phenomenex"<br />(150mm*4.6mm*5μm*80nm). HPLC show the presence in extracts of a high quantitates of<br />biologically active compounds: gallic acid and its derivatives, ellagic acid, casuarictin, catechin,<br />epicatechin and partially identified derivatives of rutin. Antioxidative activities of the extracts,<br />obtained from kernel’s pellicle, were determined in vitro by evaluation of peroxyl radical<br />scavenging capacity. Antioxidant activities were calculated in gallic acid equivalent (GAE) in<br />mM per gram of the dry residue. Antioxidant activities varied from 2.10 to 3.17 mM GAE/g, in<br />function of harvesting year and defatting pre-treatment.<br />To conclude, the pellicle of walnut kernels is a valuable source of the biologic active<br />phenolic compounds with antioxidative properties, which could be used for development of the<br />food compositions with functional properties. Notable fact: extraction of phenolic compounds<br />from the pellicle prevent a kernel’s browning and bittering. Use of so-called dephenolised<br />walnuts seems to be a promisingly way for the development of smart food technologies.</p></cfAbstr> <cfResPubl_Class> <cfClassId>eda2d9e9-34c5-11e1-b86c-0800200c9a66</cfClassId> <cfClassSchemeId>759af938-34ae-11e1-b86c-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfResPubl_Class> <cfResPubl_Class> <cfClassId>e601872f-4b7e-4d88-929f-7df027b226c9</cfClassId> <cfClassSchemeId>40e90e2f-446d-460a-98e5-5dce57550c48</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfResPubl_Class> <cfPers_ResPubl> <cfPersId>ibn-person-1537</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> <cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfPers_ResPubl> <cfPers_ResPubl> <cfPersId>ibn-person-12402</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> <cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfPers_ResPubl> <cfPers_ResPubl> <cfPersId>ibn-person-26895</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> <cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfPers_ResPubl> <cfPers_ResPubl> <cfPersId>ibn-person-37323</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> <cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfPers_ResPubl> <cfPers_ResPubl> <cfPersId>ibn-person-54161</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> <cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> </cfPers_ResPubl> <cfFedId> <cfFedIdId>ibn-doi-141881</cfFedIdId> <cfFedId>10.15414/2021.9788055224015</cfFedId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFedId_Class> <cfClassId>31d222b4-11e0-434b-b5ae-088119c51189</cfClassId> <cfClassSchemeId>bccb3266-689d-4740-a039-c96594b4d916</cfClassSchemeId> </cfFedId_Class> <cfFedId_Srv> <cfSrvId>5123451</cfSrvId> <cfClassId>eda2b2e2-34c5-11e1-b86c-0800200c9a66</cfClassId> <cfClassSchemeId>5a270628-f593-4ff4-a44a-95660c76e182</cfClassSchemeId> </cfFedId_Srv> </cfFedId> </cfResPubl> <cfPers> <cfPersId>ibn-Pers-1537</cfPersId> <cfPersName_Pers> <cfPersNameId>ibn-PersName-1537-3</cfPersNameId> <cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId> <cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFamilyNames>Tatarov</cfFamilyNames> <cfFirstNames>Pavel</cfFirstNames> </cfPersName_Pers> </cfPers> <cfPers> <cfPersId>ibn-Pers-12402</cfPersId> <cfPersName_Pers> <cfPersNameId>ibn-PersName-12402-3</cfPersNameId> <cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId> <cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFamilyNames>Иванова</cfFamilyNames> <cfFirstNames>Раиса</cfFirstNames> </cfPersName_Pers> </cfPers> <cfPers> <cfPersId>ibn-Pers-26895</cfPersId> <cfPersName_Pers> <cfPersNameId>ibn-PersName-26895-3</cfPersNameId> <cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId> <cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFamilyNames>Баерле</cfFamilyNames> <cfFirstNames>Алексей</cfFirstNames> </cfPersName_Pers> </cfPers> <cfPers> <cfPersId>ibn-Pers-37323</cfPersId> <cfPersName_Pers> <cfPersNameId>ibn-PersName-37323-3</cfPersNameId> <cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId> <cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFamilyNames>Sandu</cfFamilyNames> <cfFirstNames>Iuliana</cfFirstNames> </cfPersName_Pers> </cfPers> <cfPers> <cfPersId>ibn-Pers-54161</cfPersId> <cfPersName_Pers> <cfPersNameId>ibn-PersName-54161-3</cfPersNameId> <cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId> <cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId> <cfStartDate>2021T24:00:00</cfStartDate> <cfFamilyNames>Brindza</cfFamilyNames> <cfFirstNames>Ján</cfFirstNames> <cfFamilyNames>Бриндза</cfFamilyNames> <cfFirstNames>Я.</cfFirstNames> </cfPersName_Pers> </cfPers> <cfSrv> <cfSrvId>5123451</cfSrvId> <cfName cfLangCode='en' cfTrans='o'>CrossRef DOI prefix service</cfName> <cfDescr cfLangCode='en' cfTrans='o'>The service of issuing DOI prefixes to publishers</cfDescr> <cfKeyw cfLangCode='en' cfTrans='o'>persistent identifier; Digital Object Identifier</cfKeyw> </cfSrv> </CERIF>