Climate change, frequent droughts, abrupt changes in weather conditions during the season, as well as in connection with this the growth of phytopathogenic diseases, have become significant problems for the cultivation of grain crops. One of the methods that allow withstanding adverse weather conditions is the treatment of seeds before planting. It is directed not only at increasing and accelerating germination, but also at obtaining a high and qualitative harvest. Currently, various methods of seed processing are known using natural biologically active microbial preparations, especially from yeasts. Results about the influence of preparations of levurian origin on the indices of plant growth and development are described in the literature, but specifically about the effect of mannoprotein preparations in this regard, data have not been found. In this context, the research aimed to test the mannoprotein preparation LB-MP in the concentration of 10 mg/mL at the ability to stimulate seed germination, growth, and development of Triticosecale Wittm plants, Ingen 40 variety. The biologically active preparation based on yeast biomass from brewing waste from the Kellers brewery, Budeshti was obtained through several stages: autolysis of yeast biomass with sodium phosphate buffer, subsequent processing of the remaining biomass with 1N NaOH for 2 hours at a temperature of +80 ±5 °C, obtaining the alkaline extract and sedimenting the mannoproteins with ethyl alcohol. The seeds were treated with LB-MP mannoprotein preparation in the concentration of 10 mg/mL and its distilled water solutions of 0.33; 0.5; 1; 2 and 10 %/V. Distilled water was served as a control. The experiments were performed at room temperature +23–24 °C, in three repetitions. Three days after germination, the following parameters were evaluated: the formed number of roots, the length of the roots and stem, the dry mass of the roots and stem of each plantule. The obtained results indicate that the treatment of seeds with LB-MP bio preparation in the concentration of 10 mg/mL increases the number of roots by 6 %, the length of the roots by 10 %, the dry mass of roots and stems by 4–11 % compared to the control sample. The stimulating effect was also established in the case of seed processing with the aqueous solution of the LB-MP preparation in the concentration of 0.5 %. The results indicate a 10 % increase in the number of roots, a 5 % increase of stem length, a dry mass of roots and stems by 6–10 %, compared to the control variant. The other concentrations of the preparation had a neutral influence, the values of the studied indices oscillating at the control level or had a negative effect. Thus, taking into account the established effect and the fact that the preparation can be obtained at a convenient price from the waste of the beer industry, research on the use of yeast preparations as stimulants of crop plants is perspective.
|