This paper reveals the peculiarities of spatial distribution of Dnester River ihtyocenosis under the influence of limiting anthropogenic factors. In condition of multiple hydrobiotope fragmentations of Denster River have caused significant changes in hydrological, thermal, hydrochemical and hydrobiological regimes. They were affected characteristic habitats of fish species in the wetlands and that rheophyls oxyphyls, litho - psammophyilous. On the background decimation diversity of endemic stenobiont freshwater fish species are found progression of euritope, euritherm and mixohalinous taxon which now actively contribute to the pontisation process and limniphycation of Dnester River ichtyofauna.