Two species of the potato cyst nematodes (PCN) Globodera rostochiensis (Wollenweber) Behrens and Globodera pallida(Stone) Behrens are considered as major quarantine pests of the potato crop in temperate regions of the world which could reduce potato yield from 20 to 70%. Therefore control of these plant pests is essential. The control is generally based on the use of resistant cultivars, crop rotation, cultural practices or chemical nematicides. Although chemical nematicides successfully control plant parasitic nematodes and in practice are frequently used, this control method is generally considered dangerous for nature, beneficial soil organisms and human and animal health. For this reason interest in alternative nematode control methods has grown rapidly during recent years. In this study, the effects of vermicompost with Eisenia fetida on the development and survival of the potato-cyst nematodes G. rostochiensis (pathotype Ro1) and G. pallida (pathotype Pa2) were evaluated as alternative control method to chemicals. Solid vermicompost derived from municipal green wastes (30% leaves and 70% grass) with the addition of E. fetida earthworm. Soil amended with this material at different dosages (10, 20, 40 and 60 t/ha) significantly decreased the number of cysts/100 g dried soil, the number of eggs and juveniles/ cyst and the number of eggs and juveniles/g soil of both nematode species in comparison to the untreated controls(0 t/ha) (Table 1). The suppressive effect significantly increased with the increase of treatment dose. G. rostochiensis was more sensitive to all the tested doses than G. pallida. Cyst-forming nematodes invade plant roots as infective J2s that hatch from eggs retained in protective cysts. Treatment with solid vermicompostisonly possible prior to potato sowing. Thisvermicompost represent a new promising control method of potato-cyst nematodes in sustainable agricultural systems. Aknowledgement: This study was supported by grant Vega 2/0013/16 (0.2), project “Centrum of Excellence for Parasitology“ (ITMS 212 Code: 26220120022) based on the support of the Operational Programme “Research & 213 Development” funded from the European Regional Development Fund (0.8) and the agreement between CNR (Italy) and A.S.M. (R. Moldova).