The article presents the results of the study of maternal ancestors in terms of productivity for the highest lactation, the productivity of their offspring for the first lactation and the realization by them of the genetic potential of the Holstein breed of Ger-man, French and Dutch selection. The aim of the research was to assess the milk productivity of female ancestors and the realization of the genetic potential of the Holstein breed of various origins. In SLL "Total Gnatyuk", the implementation of the genetic potential for milk yield was higher at the first heifers of the French breed and amounted to 86.2%, which is by 15.3% more than the average of the first heifers of the German breed (70.9%).In SLL "Das-tocom", the realization of the genetic potential of the first heifers of German breed in terms of milk yield was 83.6%, in terms of fat content - 100.7%.In SLL "Holstein", the realization of the genetic potential of French breed of first heifers was 67.8%, in terms of fat content - 102.8%.In SLL "Heximsud", the realization of the genetic potential of Dutch heifers in terms of milk yield was 81.8%, in terms of fat content - 90.8%.