The use of interbreeding along with purebred breeding will make it pos-sible to obtain, in addition to the genetic improvement of many breeding traits of animals, a significant level of variability, which is mandatory for successful breeding. In view of the above, the aim of our work was to investigate the variability of se-lection traits in cows of the Ukrainian Black-and-White dairy breed depending on different methods of their breeding. It was established that the formation of economically useful traits in animals de-pended on the methods of their breeding. Thus, in terms of live weight and average daily growth during the breeding period, signs of the reproductive capacity of first-calving cows, the level of milk yield and the amount of milk fat, cows obtained from purebred breeding turned out to be the best, and in terms of milk fat - animals obtained from the crossing of cows of Ukrainian selection with bulls of the Holstein breed. The influence of breeding methods on milk productivity of cows, depending on the trait and lactation, ranged from 2.3 to 13.8%.