Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
186 0 |
SM ISO690:2012 BIBIRE, Nela, VIERIU, Madalina, PANAINTE, Alina Diana, AGOROAEI, Luminiţa, UNCU, Livia, VLASE, Cristina Victorina, VLASE, Aurel. A new high performance liquid chromatographic analysis method for ciprofloxacin. In: Revista de Chimie, 2015, vol. 66, supl. nr. 9, pp. 1463-1466. ISSN 0034-7752. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Revista de Chimie | |
Volumul 66, Supliment nr. 9 / 2015 / ISSN 0034-7752 | |
|
|
Pag. 1463-1466 | |
Rezumat | |
A rapid and simple high performance liquid chromatographic method for the quantitatiue analysis of ciprofloxacin has been developed and validated. The separation was performed on an Agilent 1100 system equipped with a ZorbaxXDB-CN (100mm x 4.6mm) column using a mobile phase consisting of a mixture of phosphoric acid solution and acetonitrile (90:10, v/v), at a flow rate of 0.8mL/min. The sample volume injected was 5μL. The retention time for ciprofloxacin was 1.8 min and the overall time of the analysis was 2.2 min. The detection was done spectrophotometrically at 278nm. The limit of quantification was 0.28/μg/mL. Calibration graphs were linear for 0.28-0.70μg/mL of ciprofloxacin, with a correlation coefficient of 0.998. The average recoveries for ciprofloxacin and coefficients of variation were 99.31% and 1.30% respectively. The new method was sensitive, specific and reproducible, suitable for the quantitative analysis of ciprofloxacin in various pharmaceuticals products. |
|
Cuvinte-cheie ciprofloxacin, liquid chromatography, validation |
|
|