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618.31-006.882-07-036 (1) |
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![]() GRECICHINA, Ecaterina, FULGA, Veaceslav, SINIŢÎNA, Lilia, GLOBA, Tatiana, DAVID, Valeriu. Evaluation of BCL2 expression in early hydatiform mole. In: Revista de Ştiinţe ale Sănătăţii din Moldova, 2022, nr. 3 An.1(29), p. 24. ISSN 2345-1467. |
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Revista de Ştiinţe ale Sănătăţii din Moldova | |||||||
Numărul 3 An.1(29) / 2022 / ISSN 2345-1467 | |||||||
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Background. The gestational trophoblastic disease is a heterogeneous group of trophoblast proliferative lesions involving cyto-syncitiotrophoblast. The establishing of a marker by immunohistochemical investigations could influence the diagnostic of molar pathology. Objective of the study. Evaluation of anti-BCL2 immunoexpression in the epithelial compartment of the villous trophoblast in the hydatidiform mole. Material and Methods. Abortion product from 15 patients diagnosed with hydatidiform mole by ultrasound and morphological imaging with anti-p57 (NCL-L-p57, clone 25B2, dilution: 1: 100, incubation period 30 min) and marker for Bcl2 protein: anti-Bcl2 (oncoprotein, clone 124, incubation period 25 min). Detection system: NovolinkTM MaxPolymer (RE7280-K, Leica). Results. The hydatidiform mole was distributed according to the differentiated antip57 immunoexpression in the villous trophoblast into: MHC - 8 cases (53.35%) vs MHP - 7 cases (46.6%). Immunoexpression in the villous trophoblast was as follows: the positive reaction was attested in 100% of cases in the syncytiotrophoblastic component, and in the cytotrophoblast it was negative. The endometrial (immunopositive) glands served as a positive internal control. Negative control - replacement of the primary antibody TRIS-buffer saline. Conclusion. Differentiated immunoexpression of BCL2 protein in the trophoblastic compartment of chorionic villi allows the evaluation of the trophoblastic epithelial profile in gestational molar pathology. |
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Cuvinte-cheie anti-BCL2, hydatidiform mole, molar pregnancy, trophoblastic disease, anti-BCL2, mola hidatiformă, sarcină molară, boală trofoblastică |
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<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xmlns='http://datacite.org/schema/kernel-3' xsi:schemaLocation='http://datacite.org/schema/kernel-3 http://schema.datacite.org/meta/kernel-3/metadata.xsd'> <creators> <creator> <creatorName>Grecichina, E.V.</creatorName> <affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation> </creator> <creator> <creatorName>Fulga, V.M.</creatorName> <affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation> </creator> <creator> <creatorName>Siniţîna, L.A.</creatorName> <affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation> </creator> <creator> <creatorName>Globa, T.G.</creatorName> <affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation> </creator> <creator> <creatorName>David, V.B.</creatorName> <affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation> </creator> </creators> <titles> <title xml:lang='en,ro'>Evaluation of BCL2 expression in early hydatiform mole</title> </titles> <publisher>Instrumentul Bibliometric National</publisher> <publicationYear>2022</publicationYear> <relatedIdentifier relatedIdentifierType='ISSN' relationType='IsPartOf'>2345-1467</relatedIdentifier> <subjects> <subject>anti-BCL2</subject> <subject>hydatidiform mole</subject> <subject>molar pregnancy</subject> <subject>trophoblastic disease</subject> <subject>anti-BCL2</subject> <subject>mola hidatiformă</subject> <subject>sarcină molară</subject> <subject>boală trofoblastică</subject> <subject schemeURI='http://udcdata.info/' subjectScheme='UDC'>618.31-006.882-07-036</subject> </subjects> <dates> <date dateType='Issued'>2022-10-21</date> </dates> <resourceType resourceTypeGeneral='Text'>Journal article</resourceType> <descriptions> <description xml:lang='en' descriptionType='Abstract'><p>Background. The gestational trophoblastic disease is a heterogeneous group of trophoblast proliferative lesions involving cyto-syncitiotrophoblast. The establishing of a marker by immunohistochemical investigations could influence the diagnostic of molar pathology. Objective of the study. Evaluation of anti-BCL2 immunoexpression in the epithelial compartment of the villous trophoblast in the hydatidiform mole. Material and Methods. Abortion product from 15 patients diagnosed with hydatidiform mole by ultrasound and morphological imaging with anti-p57 (NCL-L-p57, clone 25B2, dilution: 1: 100, incubation period 30 min) and marker for Bcl2 protein: anti-Bcl2 (oncoprotein, clone 124, incubation period 25 min). Detection system: NovolinkTM MaxPolymer (RE7280-K, Leica). Results. The hydatidiform mole was distributed according to the differentiated antip57 immunoexpression in the villous trophoblast into: MHC - 8 cases (53.35%) vs MHP - 7 cases (46.6%). Immunoexpression in the villous trophoblast was as follows: the positive reaction was attested in 100% of cases in the syncytiotrophoblastic component, and in the cytotrophoblast it was negative. The endometrial (immunopositive) glands served as a positive internal control. Negative control - replacement of the primary antibody TRIS-buffer saline. Conclusion. Differentiated immunoexpression of BCL2 protein in the trophoblastic compartment of chorionic villi allows the evaluation of the trophoblastic epithelial profile in gestational molar pathology.</p></description> <description xml:lang='ro' descriptionType='Abstract'><p>Introducere. Boala trofoblastică gestațională constituie un grup eterogen de leziuni proliferative ale trofoblastului cu implicarea cito-sincițiului trofoblastic. Stabilirea unui marker prin investigații imunohistochimice ar putea influența particularitățile diagnostice a patologiei molare. Scopul lucrării. Evaluarea imunoexpresiei anti-BCL2 în compartimentul epitelial trofoblastic vilos în mola hidatiformă. Material și Metode. Produsul avortic de la 15 paciente diagnosticate cu molă hidatiformă prin imagistica ecografică și morfologică cu anti-p57 (NCL-L-p57, clona 25B2, diluția: 1:100, perioada de incubare 30 min) și markerul pentru proteina Bcl2: anti-Bcl2 (oncoprotein, clona 124, perioada de incubare 25 min). Sistemul de detecție: NovolinkTM MaxPolimer (RE7280-K, Leica). Rezultate. Mola hidatiformă a fost repartizată conform imunoexpresiei diferențiate anti-p57 la nivelul trofoblastului vilos în: MHC – 8 cazuri (53,35%) vs MHP – 7 cazuri (46,6%). A fost evaluată imunoexpresia la nivelul trofoblastului vilos: reacția pozitivă a fost atestată în 100% cazuri în componenta sincițiotrofoblastică, iar în citotrofoblast a fost negativă. Drept control intern pozitiv au servit glandele endometriale (imunopozitive). Control negativ – substituirea anticorpului primar TRIS-buffer saline. Concluzii. Imunoexpresia diferențiată a proteinei BCL2 în compartimentul trofoblastic al vilozităților coriale permite evaluarea profilului epitelial trofoblastic în patologia molară gestațională.</p></description> </descriptions> <formats> <format>application/pdf</format> </formats> </resource>