The aim of the present study was to determine the chemical composition of the essential oil and the evaluation of the antimicrobial activity of ethanolic extract from T. corymbosum plants from the Republic of Moldova. Hydrodistillation and Soxhlet extraction have been used to obtain volatile oil samples and extracts from plants, respectively. The components of volatile oil were identified by GC-MS analyses. The antibacterial activity of the extracts was assessed by the successive double dilution method. The GC-MS analysis revealed the presence of 38 compounds, including terpenes germacrene D (33.3% and 47.5%), (Z)-β-farnesene (8.6% and 16.1%), -elemene (3.1% and 5.2%), β-caryophyllene (4.2% and 6.5%), aliphatic - palmitic (2.2% and 7.1%) and linoleic (0.2% and 1.0%) fatty acids, fatty alcohol n-octadecanol (0.6% and 9.7%), higher alkane n-heneicosane (1.0% and 6.9%) as the major constituents. The in vitro assessments of hydroacoholic extract against five bacterial strains and two fungal species showed its promising antibacterial/antifungal activities at 0.03% and 0.015%, respectively. According to the obtained data, the T. corymbosum species that grows in Moldova belongs to the germacrene D chemotype. This species holds great potential to be used as an herbal antibacterial agent.