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SM ISO690:2012 DASCALIUC, Alexandru, CĂLUGĂRU-SPĂTARU, Tatiana, PARII, Yaroslav, PARII, Yulia. In vivo and in vitro control of Rhodiola rosea L. plants growth and accumulation of secondary metabolites. In: Agrobiodiversity for improving the nutrition, health, quality of life and spiritual human development, 3 noiembrie 2021, Nitra. Nitra, Slovacia: Slovak University of Agriculture in Nitra, 2021, Ediția a 5-a, p. 40. ISBN 978-80-552-2401-5. 10.15414/2021.9788055224015 |
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Agrobiodiversity for improving the nutrition, health, quality of life and spiritual human development Ediția a 5-a, 2021 |
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Conferința "Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and Spiritual Human Development" Nitra, Slovacia, 3 noiembrie 2021 | |||||||
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Our research aimed to determine the effect of some physical and chemical factors on the |
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Cuvinte-cheie Rhodiola rosea, in vivo, in vitro, secondary metabolites |
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<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xmlns='http://datacite.org/schema/kernel-3' xsi:schemaLocation='http://datacite.org/schema/kernel-3 http://schema.datacite.org/meta/kernel-3/metadata.xsd'> <identifier identifierType='DOI'>10.15414/2021.9788055224015</identifier> <creators> <creator> <creatorName>Dascaliuc, A.P.</creatorName> <affiliation>Institutul de Genetică, Fiziologie şi Protecţie a Plantelor, Moldova, Republica</affiliation> </creator> <creator> <creatorName>Călugăru-Spătaru, T.N.</creatorName> <affiliation>Institutul de Genetică, Fiziologie şi Protecţie a Plantelor, Moldova, Republica</affiliation> </creator> <creator> <creatorName>Parii, I.</creatorName> <affiliation>Всеукраинский институт растениеводства, Ucraina</affiliation> </creator> <creator> <creatorName>Parii, I.</creatorName> <affiliation>Всеукраинский институт растениеводства, Ucraina</affiliation> </creator> </creators> <titles> <title xml:lang='en'>In vivo and in vitro control of Rhodiola rosea L. plants growth and accumulation of secondary metabolites</title> </titles> <publisher>Instrumentul Bibliometric National</publisher> <publicationYear>2021</publicationYear> <relatedIdentifier relatedIdentifierType='ISBN' relationType='IsPartOf'>978-80-552-2401-5</relatedIdentifier> <subjects> <subject>Rhodiola rosea</subject> <subject>in vivo</subject> <subject>in vitro</subject> <subject>secondary metabolites</subject> </subjects> <dates> <date dateType='Issued'>2021</date> </dates> <resourceType resourceTypeGeneral='Text'>Conference Paper</resourceType> <descriptions> <description xml:lang='en' descriptionType='Abstract'><p>Our research aimed to determine the effect of some physical and chemical factors on the<br />synthesis and accumulation of secondary metabolites in Rhodiola rosea L. plants grown in vivo<br />and in vitro. To achieve this goal, we have identified the following objectives: 1. To elaborate on<br />the agrotechnical regime of R. rosea plants growth during cultivation artificially and in the field;<br />2. To determine the heterogeneity of R. rosea plants by analyzing morphological, physiological,<br />and biochemical parameters; 3. To analyze the influence of UV radiation and other physical and<br />chemical factors on the accumulation of secondary metabolites in R. rosea plants grown in the<br />laboratory, greenhouse, and field conditions in Moldova.<br />We have collected seeds from R. rosea plants grown in the Carpathian Mountains and<br />elaborated optimal conditions for their cultivation in vitro and in vivo. We developed sensitive<br />HPLC methods for quantitative and qualitative analysis of secondary mнекуцйetabolites.<br />We performed experiments with the R. rosea plants obtained from seeds collected in the<br />Carpathian Mountains and with the plants propagated in vitro. The results showed that the<br />received plants differ by their morphology and growth rate. The content of secondary<br />metabolites in rhizomes from plants cultivated in the greenhouse and field conditions was<br />lower than that of those collected in the mountains. After periodic illumination of plants with<br />ultraviolet radiation and their treatment with the biostimulator Reglalg, the content of<br />secondary metabolites in rhizomes increased to a level comparable to those in rhizomes<br />collected in the Carpathian Mountains. The introduction of the biostimulator Reglalg in the<br />culture medium, and the exposure of R. rosea callus in the logarithmic growth phase at 4oC,<br />during 12 hours, stimulated the accumulation of secondary metabolites in callus biomass. We<br />selected a vigorous clone of plant induced from the cells of R. rosea callus and transferred it for<br />propagation in vivo. The generative descendants of this clone served as initial material for the<br />selection of the R. rosea cultivars Tatiana, certified in Ukraine.<br />The research conducted allowed elucidating and optimizing the combined influence of the<br />main factors that determine the growth and accumulation of secondary metabolites in the in<br />vitro and in vivo culture of R. rosea. More promising results were obtained with the plants<br />cultivated under in vivo conditions. Currently, using accumulated information, we are<br />developing economically advantageous technologies for artificially large-scale cultivation of the<br />R. rosea plants that contain high levels of secondary metabolites in their rhizomes.</p></description> </descriptions> <formats> <format>application/pdf</format> </formats> </resource>