We focused our research on testing the assumption that the response of biological
systems to stressors depends on the general or similar effects of various adaptogens on the
viability and health of humans, animals, and plants due to the available mechanisms of response
to factors inherited during evolution. We present the results obtained in studying the influence
of biostimulators, extreme temperatures, and ultraviolet radiation on the accumulation of
secondary metabolites in Rhodiola rosea L., cultivated in conditions in vivo and in vivo.
In research, we used golden root plants collected from the Carpathian Mountains or
artificially grown in vivo and in vitro. In laboratory and field conditions, we determined the
influence of different physical and chemical factors on the growth, accumulation, and
composition of secondary metabolites in the in vivo and in vitro culture of the golden root. In
parallel, tested the influence of substances with the property's characteristic for adaptogens on
the resistance and productivity of higher plants to the action of extreme temperatures and
gamma radiation.
We developed and adjusted the conditions for multiplying and obtaining golden root
biomass from plants cultivated in vivo and the callus growing in vitro. The content of secondary
metabolites in rhizomes and callus biomass was lower than in plant rhizomes collected in the
mountains. After introducing un culture medium the precursors of secondary metabolite, or
biostimulators with adaptogen properties, the accumulation of secondary metabolites in callus
cells of the golden root increased. Short-term exposure of callus to low temperatures and
ultraviolet radiation assured a similar beneficial effect on the accumulation of secondary
metabolites. The treatment with biostimulators that demonstrate adaptogen properties of the
seeds and different species of plants by vegetation lead the increasing plants primary and
adaptive resistance to heat, frost, and gamma radiation. The use of biostimulators to treat wheat
seeds before sowing, depending on the conditions of the year and variety, ensured the harvest
increased by 0.2–1.2 tons per hectare.
The modification plants' response to the action of abiotic stresses using biostimulators
and adaptogens is similar. Developing efficient cultivation methods and accumulation of
secondary metabolites by golden root plants grown in vitro or in vivo opens new possibilities
for obtaining raw materials in sufficient quantities for the large-scale and economically
advantageous implementation of adaptogens in medicine and organic agriculture.