The trophic spectrum of a family of long-eared owl in the northern part of the Republic of Moldova during nesting period was analyzed. The pellets were collected at the end of reproductive period from the nest and under it. A total of 59 pellets were analyzed. The mean number of individuals per pellets was 2.14, minimum – 1, maximum – 5, the size of pellets varied between 0.7cm and 4.5 cm. The main prey of Asio otus individuals was Microtus genus species (M. arvalis and M. rossiameridionalis), which constituted 73%, followed by species of genera Mus (M. spicilegus and M. musculus) and Apodemus (A. sylvaticus, A. uralensis and A. flavicollis). There was found one individual of Muscardinus avellanarius, which constituted less than 1%. Thus, the trophic spectrum of long-eared owl in breeding period consist mostly of field vole, which is the most abundant in agroecosystems and is considered pest species.