Introduction. One of the most current and complicated aspects of plastic surgery is the treatment of defects in diabetic patients. The inefficiency of the conservative treatment and the the surgical interventions with the sacrifice of other healthy tissues requires the use of new methods of treatment Aim of the study. Optimizating the local regeneration in patients with peripheral circulatory disorders using biological materials obtained through tissue engineering Materials and methods. We initiated a study in a group of 5 patients with peripheral circulatory disorders with chronic non-healing wounds (> 30 day duration). The pacients were selected to evaluate the performance, safety and handling properties of dehydrated human amnion / chorion membrane allograft. All five patients received only one application of dehydrated human amniotic membrane and there were no adverse effects. Results. Was obtained optimization of local regeneration in patients with peripheral circulatory disorders by tissue engineering methods Conclusions. The research in this direction will establish the perfect combination of support material for cells and growth factors, for a faster and qualitative epithelialization, thus facilitating epithelialization of ulcers and wounds in patients with circulatory disorders