One of the main problems of modern vegetable farming is the creation of new economically efficient varieties with complex valuable characters adapted to different growing conditions. In tomato breeding, the genotypes that possess the j-2 and j-2in (jointless-2) genes are particularly important, because the fruits of such plants are easier detached from the peduncle and are less deformed during harvesting and transportation. The presence of peduncles causes the decrease of fruit quota and reduction of product quality, conferring them a bitter taste. In order to reveal the variability of agronomical traits and specify the value of tomato genotypes without geniculate joint of floral peduncle, a comparative assessment was carried out based on a range of useful characters. The varieties were evaluated in terms of the most valuable biological parameters (yield, fruit weight, fruit quota, vegetation period, pericarp thickness, cold and draught resistance). Genotypes characterized by both precocity and high productivity were identified in the collection of cultivated tomatoes. The analysis of tomato varieties based on resistance to cold permitted highlighting of highly resistant genotypes, which may be used as initial material for breeding purposes.