Problem-based learning (PBL) is an approach that challenges students to learn through engagement in real problems. But it is important to take into consideration that this method assumes changing not only the teaching strategy, but the main beliefs and values from the universities. Thus in order to implement this method at the universities from the Republic of Moldova we should assure that the organizational culture from higher educational system existent now in Moldova is appropriate. The purpose of this article is to explore the concept of current organizational culture at education institutes so the effective management methods will be developed. The analysis of the organizational culture’s dimensions allows observing human behavior within the universities and high lighting reality, identifying the strengths and also the weaknesses which have an impact on its functionality and development. In this paper, we try to present some models for assessing organizational culture in universities for the reason of implementing PBL.