Methods of DNA Analysis
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NAFFAR, Said, HINDI, Nadine. Methods of DNA Analysis. In: NANO-2019: Limits of Nanoscience and Nanotechnologies, Ed. 2019, 24-27 septembrie 2019, Chişinău. Chișinău, Republica Moldova: 2019, p. 63.
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NANO-2019: Limits of Nanoscience and Nanotechnologies 2019
Conferința "SPINTECH Summer school “S/F Hybrid Structures for Spintronics”"
2019, Chişinău, Moldova, 24-27 septembrie 2019

Methods of DNA Analysis


Pag. 63-63

Naffar Said, Hindi Nadine
 
”Nicolae Testemițanu” State University of Medicine and Pharmacy
 
 
Disponibil în IBN: 27 ianuarie 2020


Rezumat

Methods of DNA analysis are methods which are used to process and engineer DNA. These methods are most commonly used in the field of genetic engineering. They allow us to cut and rebuild segments of DNA, replicate small samples of DNA (forensic investigations) and generally provide a way to treat genetic diseases. This topic was chosen for this report because of the high potential it has in making an innovative change in the healthcare system. The methods of DNA analysis are 3 in number and each method is used for a certain cause. The first method is the southern blot: southern blot is a method for finding the lengths of DNA strands using constriction enzymes to cut a long DNA strand into smaller pieces and then these pieces are put in gel electrophoresis to determine the lengths of the new strands. This method helps to determine mutations in the DNA based on the number and the length of the new DNA strands. The second method is DNA sequencing: this method is used for determining the order of nucleotides in the DNA and thus determining the information which the DNA codes for. The third method is PCR (polymerase chain reaction) - this method is used to amplify small samples of DNA into large amounts for the cause of finding mutations or for testing of a DNA sample which was found at a crime scene. This method is based on primers which determine the length of the amplified DNA (from where to where). It is basically the same method which our cells use to replicate DNA, only that it’s much faster and it is done in vitro. In conclusion, the methods of DNA analysis are useful methods for the investigation of DNA. They provide us with the ability to engineer our DNA to prevent or cure certain diseases and syndromes. And they are an important part of the healthcare industry’s way to develop and find new ways for curing and preventing diseases.