Rapid prototyping techniques are a very interesting group of methods for the preparation of substrates for tissue engineering and regenerative medicine. Firstly, they allow for a personalized approach to the defect of the patient, and secondly, they allow the microstructure to be optimized for the needs and requirements of the cells of substituted tissue [1-2]. Limitations however remain linked to the materials which can be used to print the mentioned scaffolds. The neat polymers (e.g. polylactide) are inert to the living cells (they do not stimulate them to differentiate or release proteins that stimulate the adhesion). In turn, attempts to obtain composite monofilament, e.g. using bioactive ceramics, are ineffective. Difficulties with dispersion of filler cause unstable cross-section of monofilament and prevent printing [3]. This research attempted to obtain bioactive scaffolds for bone tissue engineering using the 3D printing of the inert PLA. Scaffolds with various pore geometry (hexagonal and square) and 60% filling were subjected to the two-stage functionalization of the surface. In the first stage, the scaffold was etched for 24h in 1M NaOH and then the bioactive layer (hydroxyapatite HAp, carbon nanotubes CNT and their mixtures) was applied onto the structure using the EPD method. As a positive control in bioactivity tests (incubation in simulated body fluid SBF), a scaffold with a nanoparticle hydroxyapatite (Sigma Aldrich) was used. The scaffolds prepared without etching but obtained by EPD were also tested for bioactivity. Both series of scaffolds with applied layers (etched and not-etched) were compared for microstructure (functionalized scaffolding surface), thermal stability TG/DSC, bioactivity and adhesion at the substrate-layer joints (tape test). The etching step has been shown to provide a homogeneous layer of both HAP and CNT. The etching process does not affect the thermal stability of the scaffold. All the scaffolds exhibit bioactivity: etched after 7 days, not-etched after 21 days incubation in SBF. Presented approaches may be an interesting alternative to applications for personalized implants with complex shapes and filling.