Genes cloned from diverse plants for different pathogens resistance have the structural similarity presumably involved in pathogen recognition and signal transduction in triggering the defence response. The RGA technique offers some advantages for identify of candidate disease resistance genes at different plant species, provides to develop informative markers for assessing the genetic diversity of germplasm and also simplify plants disease resistance breeding programs. The purpose of the present research was to examine the germplasm diversity and describe genetic relationships among the investigating genotypes of chickpeas based on several conserved domains of RGAs. Three desi (s. Botna, MDI02416, MDI02432) and three kabuli (s.Ichel, MDI02419, MDI02440) chickpea genotypes with a different level of productivity and diseases resistance were used in this study. Six pairs of RGA primers based on conserved leucinerich repeats (NLRR for/rev for N gene conferring resistance to TMV, CLRR for/rev - Cf9 gene resistance to Cladosporium fulvum, XLRR for/rev - Xa21 gene resistance to Xanthomonas campestris pv oryzae, RLRR for/rev - RPS2 gene resistance to Pseudomonas syringae) and serine/threonine protein kinases domains (Ptokin1/2 and Ptokin3/4 resistance to bacterial pathogen Pseudomonas syringae pv tomato) of several resistance genes were used. As a result of genotyping by means of RGA markers it was found that the investigation genotypes are quite heterogeneous. The use of RGA primers has allowed to reveal specific and polymorphic fragments in chickpea. Primers designed based on the conserved domains of resistance genes often amplify multiple fragments that is also observed in the given experience. RGA-analysis has revealed in most cases 4-12 amplicons corresponding to candidate resistance genes (except for RLRR for/rev, CLRR for/rev and Ptokin 3/4 primers, which were not yield any amplicon). The primers Ptokin1/2 produced the highest level of polymorphism (62.5%), at the time as level of polymorphism by means of primers XLRR for/rev, NLRR for/rev and Ptokin1/2 averaged 29.17%. To infer genetic relationships among the investigated genotypes dendrograme based on the RGA fragments was constructed using Nearest Neighbor method, Euclidean distances. According to the similarity level, the genotypes were divided in 2 clusters: cluster 1 is formed of s.Botna, MDI02432, s.Ichel, MDI02440 and cluster 2 – MDI02416, MDI02419. In conclusion, out of tested 6 pair of primers only 3 pairs were effective in distinguishing of the genotypes for different pathogens resistance. It is vital to note that polymorphism detected in chickpea may result from different mutations within the conserved domains but at the same time the amplicons may represent candidate genes for disease resistance or other important signal-transduction processes. The RGA markers have allowed to group together the genotypes with higher level of resistance forming a single cluster 1, this information will be highly relevant for the selection of genotypes for the breeding and deployment of disease resistance genes.