Approach to stabilization of the human chorionic gonadotropin activity
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611.013. (10)
Anatomy. Human and comparative anatomy (240)
SM ISO690:2012
SLYVCHUK, Yurij, HEVKAN, Ivan, MATIUKHA, Iryna, SHTAPENKO, Oksana, FEDOROVA, Svitlana, TKACHENKO, Halyna, KURHALUK, Natalia. Approach to stabilization of the human chorionic gonadotropin activity. In: Medicină veterinară: Lucrări ştiinţifice, 20 septembrie 2019, Chişinău. Chişinău Republica Moldova: Centrul editorial UASM, 2013, Vol.35, pp. 84-88. ISBN 978-9975-64-247-7.
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Medicină veterinară
Vol.35, 2013
Simpozionul "Simpozionul Ştiinţific Internaţional „Agricultura Modernă – Realizări şi Perspective” consacrat aniversării de 80 de ani de la Înfiinţarea Universităţii Agrare de Stat din Moldova"
Chişinău, Moldova, 20 septembrie 2019

Approach to stabilization of the human chorionic gonadotropin activity

CZU: 611.013.

Pag. 84-88

Slyvchuk Yurij1, Hevkan Ivan1, Matiukha Iryna1, Shtapenko Oksana1, Fedorova Svitlana1, Tkachenko Halyna2, Kurhaluk Natalia2
 
1 Institute of Animal Biology, National Academy of Agrarian Science,
2 Institute of Biology and Environmental Protection, Pomeranian University
 
 
Disponibil în IBN: 6 iunie 2019


Rezumat

The aim of our study was a comprehensive search of an optimum quantitative and qualitative composition of organic compounds needed for the stabilization of the human chorionic gonadotropin (hCG). As a result of the conducted investigations, it has been established that, with the samples of chorionic gonadotropin containing L-lysine kept in a thermostat at a temperature of 40°C during 8 weeks, the most stable concentration of the gonadotropin was found in the samples of the experimental series containing 10 mg/cm3 of L-lysine – over 54 per cent of the initial theoretic concentration of hCG. In the conditions of a prolonged conservation of the gonadotropin in the samples containing L-glycine, variations of the concentration of native gonadotropin are observed in all series of samples; yet the final results demonstrate that L-glycine added in the quantity of 0.2 mg/cm3 has the most pronounced stabilizing effect. Conservation of samples containing 0.2 mg/cm3 of L-methionine provides a relatively high and stable activity of gonadotropin during 6 weeks of conservation. The concentration of native hCG in this series of samples during this period of conservation was about 50%.

Cuvinte-cheie
amino acids, Chorionic gonadotropin, Immune-chemiluminescent assay, L-Lysine, LGlycine, L-Methionine