The action of the suspensions and metabolites of microorganisms Azotobacter chroococcum and Pseudomonas fluorescens, used by application in soil when planting grape seedling and foliars, as well as the subsequent half-dose foliar fertilization by complex of trace Microcom-V, on the mineral nutrition of grapes, was studied. It was established that the application of the suspension of microorganisms P. fluorescens + A.chroococcum together with the halved dose Microcom-V increases the availability of nutrients for plants, the activity of some metabolic processes, grows and development of seedlings, promotes improving the quality of planting material.