In vitro testing in response to selective biotic and abiotic factors is determined by the existence of positive relations between the sensitivity or tolerance of integral plants in vivo and of explants in vitro [1, 2, 3]. The tissue crop technique is considered a useful tool in screening of the combination ability of the genitors, choosing the best maternal form and of the hybrids within the cereal crop improvement programs [4]. Mature embryos present vitro crop with advanced morphogenetic capabilities, limitless and therefore, less expensive. The following characters were investigated: the callus frequency (%) of mature embryos of common winter wheat and callus area (mm2) at six F2 hybrids derived from the reciprocal crossover of genotypes: BT 16-04, Odeschi 267 and line L 101 in 2013 and 2014 weather conditions. As a selective factor, it was used the culture filtrate (CF) Helminthosporium avenae (Drechslera avenae Eidam.) and further, in Murashige-Scoog (MS) crop environment in concentration of 30% capacity.  In vitro crop witness during 2014 the callus frequency of mature embryo presented advanced levels of those manifested by studied populations of 2013 harvest conditions. During parameter occurrence, the callus frequency index BT 16-04 showed positive maternal role in combination with L 101, whereas in combination with Odeschi 267 – paternal role during 2013 and 2014.  On the background with CF H. avenae, the frequency of callus character values was at the witness level or differentially decreased. Advanced resistance showed genitors in 2014 compared to 2013. The F2 L 101 x BT 16-04 and F2 Odeschi 267 x BT 16-04 hybrids presented in both years advanced frequency to reciprocal ones. The contribution to resistance character improvement was done by the paternal factor of BT 16-04 genitor.  The callus area character for genitors attested low genotypic specificity in vitro crop witness in 2013 but higher in 2014. The reciprocal hybrids were diversified only by paternal outcome BT 16-04 in combination with the L 101 genitor in 2013 conditions, while in the 2014 – in both possible combinations.  In reaction to CF H. avenae, supplemented in environment MS (30%), the callus area character showed only repression, its variability being within the range 84.1…93.7% and 80.9…98.1% of control in 2013 and 2014, respectively. Diversifying mutual hybrids, CF H. avenae reaction occurred as a result of the paternal effect of the BT 16-04 genitor in both possible combinations in 2013 conditions, while in 2014 – only in combination with L 101 line.  The callus frequency indices settled average positive correlations in environmental conditions of the years 2013, 2014 and more advanced – in witness version compared to CF H. avenae (r=0.49 and r=0.28). The character callus area was most affected by conditions of the years, the correlations being low (r=0.03 and r=0.18, respectively, and witness CF H. avenae). The callus frequency showed major correlation with callus area within FC H. avenae under weather conditions of both years, while in the witness version – only in 2014.  Environmental conditions in 2013-2014 had considerably influenced the interaction of alleles of genes which participate in the formation of frequency characteristics and callus area of reciprocal hybrids and in their reaction to the culture filtrate Helminthosporium avenae.  During both 2014 and 2013, F2 L 101 x BT 16-04 produced increased callose area to reciprocal hybrid and the role of surface and resistance donor to fungal metabolites belonged to paternal genitor BT 16-04.