The results of  mycological analysis of genus Rosa L. ornamental shrubs infected with root and basal rots reveal that phytopathogenic complex of green cuttings’ basal part is represented by 15 species of Micromycetes from 11 genera, 7 families, 8 levels, 4 parts of 2 kingdoms. Fungi kingdom is represented by 12 species of 9 families and Chromista – by 3 species of 2 genera. Phytopathogenic complex of root and basal rots is represented by saprophytic and parasitic organisms. Saprotrofitic types of Micromycetes were distinguished from the infected Rоsa×hybrida and Rosa canina cuttings: Aspergillus spp., Trichothecium roseum, Gliocladium spp., Mucor spp., and epiphytes Alternaria spp. The allocation frequency was regular, except for species of the genus Alternaria. In some cases necrosis was detected on rooted green cuttings which contained potentially pathogenic species of Micromycetes: Botrytis cinerea, Fusarium spp., Pythium spp., Rhizoctonia solani, but the frequency of detection was low, and they were allocated irregularly. In the phytopathogenic complex of necrosis in the basal part of green cuttings Rosa × hybrida and Rosa canina, compared to other soil Micromycetes, Fusarium, Verticillium, Phytophthora species dominated. From the data presented, we should also note that the fungi F. oxysporum, F. solani, V. albo-atrum, V. dahliae, Ph. cactorum are the main participants of infection that causes rotting process in green cuttings of genus Rosa ornamental shrubs during their rooting in greenhouses. Mycological analysis of young (1-2 years) genus Rosa ornamental shrubs in the open ground, affected by root and basal rots showed that the species composition of pathogenic complex does not differ from the one set in the greenhouse for green cuttings’ rooting. The dominant place in allocation frequency is possessed by family Fusarium, the species of which were met twice as often as the Verticillium kind of pathogens and 3 times more often than the Phytophthora kind. As a result of mycological analysis of genus Rosa L. ornamental shrubs’ infected root we have found that the dominant position in a phytopathogen complex belongs to F. oxysporum, its frequency allocation is 3 times higher than F. solani. In most cases, these two pathogens complement each other. The genus Verticillium in the pathological process is represented by species of V. albo-atrum, V. dahliae, the dominant place on the frequency selection is taken by V. dahliae. The genus Phytophthora is represented by Ph. Cactorum kind. Diagnostic features of fusariose, verticilliose and phytophtoroise root and basal rot of genus Rosa ornamental shrubs are defined.