Earlier we established the possibility of using low-intensity radiation in the visible part of the spectrum to regulate the growth and biosynthetic activity of macromycetes in biotechnological processes of their cultivation. The lack of information about the dynamics of photo-induced activity in the cells of living organisms at the time was the basis for conducting studies of preservation duration on photoinduced activity of edible and medicinal mushrooms seed mycelium during storage and recultivation. The seed mycelium irradiated in the optimal regime for each strain (230 mJ/cm2) in the stationary growth phase was stored in the dark at a temperature of +50C. Sowing on a liquid nutrient medium was made immediately after irradiation and then every 24 hours. An indicator of the seed mycelium activity was the accumulation of biomass for a certain volume of the medium. As a control, a mycelium not exposed to light was used. It has been established that a significant decrease in the induced activity of the inoculum begins in the first 24 hours of storage in the species Flamulina velutipes, Hericium erinaceus and Inonotus oblicus. Seed mycelium Cordiceps militaris, Ganoderma applanatum, Pleurotus ostreatus and Lentinus edodes begin to lose activity 24 hours after irradiation. In P. ostreatus, it remains unchanged for the first 24 hours, and in G. lucidum - 48 hours. However, after 72 hours the mycelium inoculum activity in all strains decreased to control level. It will need to be taken into account when bioactivation methods by light of low intensity using in biotechnologies of macromycetes cultivation. According to the theory of universal mechanisms of photostimulation, the main physical and/or chemical changes caused by light in photoacceptor molecules are accompanied by a cascade of biochemical reactions in cells that do not require further activation by light. However, there is no information about the duration of these processes. Nevertheless, these questions are no less important in the development of high-intensive technologies for cultivation of macromycetes using artificial light than the duration of preservation of photoinduced changes after irradiation. To obtain an answer to these questions, a series of sequential passages of the photoactivated mycelium was carried out. In C. militaris, F. velutipes, G. lucidum, L. edodes and P. ostreatus strains activity level did not significantly change after two passages, whereas G. applanatum, H. erinaceus obliquus internus and P. ostreatus its decrease was observed already after the second subculture. The accumulation of mycelial biomass after inoculation of the fermentation medium with a mycelium obtained after the fourth passage after irradiation did not differ significantly from the control. This allows us to recommend the use of the photoactivated mycelium C. militaris. F. velutipes, G. lucidum, P. ostreatus and L. edodes for two consecutive inoculations, and for G. applanatum, H. erinaceus, and I. obliquus for only one.