The present research is devoted to expansion of fungicidal action of the liquid biological preparation Gliocladin-SC. This biopreparation has been included in the State Registry of phytosanitary assets and products raising the soil fertility of the Republic of Moldova under the number 08-2-0406 since 2015. The active substance in biopreparation is a fungus antagonist of phytopathogens Trichoderma virens Miller, Giddens and Foster, strain 3X. The experiments were conducted under laboratory conditions. The materials used in this study were pure cultures of fungi, which are causative agents of diseases. Pathogens were extracted from affected plants, roots and seeds of wheat, sunflower, corn, soya, peas, cabbage. The extraction was carried out by methods commonly used in microbiology (N.S.Egorov, 1995). Fungicidal activity of biopreparation was determined on agar nutrient medium in Petri dishes using the diffusion method in agar (N.S.Egorov, 2004). Incubation was done under temperature convenient for pathogens. In cases when the biopreparation exhibited antifungal activity, a sterile zone has been formed between the well with the preparation and the culture of the pathogen. As a result of the experiments, it was determined that all pathogens showed sensitivity to biological preparation to a various extent. Growth inhibition zones are shown in the table 1. Biological preparation Gliocladin-SC has a fungicidal action against pathogenic fungi, which cause Fusarium root rot of wheat, corn, sunflower, soybean, etc. It is active against pathogens affecting cabbage plants at all stages of development and during storage – R.solani, B.cinerea, T.basicola, Fusarium sp, S.sclerotiorum. It forms sterile growth inhibition zones under laboratory conditions. Application of biopreparation in agriculture will contribute to improving the phytosanitary situation in agrocenoses and obtaining environmentally friendly products.