The Plaiul Fagului Scientific Reserve is situated in the North-West of the Central Moldavian Hills, 70 km away from Chişinău, at approximately 47°17’28”N 28°3’16”E in the Central Region of the Republic of Moldova. This reserve is an important part of the Moldavian protected areas with a total surface of 5,558.7 sq km. Several rare species of insects are mentioned here, 39 of them are included in the Red Book of the Republic of Moldova (Fauna Rezervației Plaiul Fagului. Nevertebrate, 2021). Dryocoetes alni (Georg, 1856) is a xylophagous species that grows on various species of deciduous trees. Dryocoetes alni is a vector in the transmission of xylophagous fungi. The species is found in Europe, eastern and western Siberia and Turkey (Strzałka et al., 2020). The current study was aimed to isolate and identify the fungal microbiota found to be transported by bark beetles Dryocoetes alni collected from Plaiul Fagului Reserve, the Republic of Moldova. The species was collected from dead oak trees using trunk traps in the Plaiul Fagului Reserve. Microbial cultures were isolated and then purified on malt extract agar medium (MEA). Fresh fungal isolates were used for genomic DNA extraction. PCR method was used amplify the ITS1-5.8S-ITS4 DNA region prior to Sanger dideoxy sequencing. Based on the sequences similarities with other microorganisms found in the National Center for Biotechnology Information (NCBI) database, the analyzed samples were identified at specie or genus level. In our investigations, Dryocoetes alni was found to carry Querciphoma carteri (Gruyter & Boerema) Crous, named after Quercus, the host on which it occurs, morphologically similar to fungi of genus Phoma. Querciphoma carteri is known to occur in oak, causing dieback (Menkis et al., 2022). Have been reported of Q. minuta (syn. Q. carteri) as a potential canker pathogen of planetree in Germany (Boldt-Burisch & Douanla-Meli, 2023). From the Dryocoetes alni bark beetles were also isolated Alternaria alternata, A. infectoria, Cladosporium cladosporioides, C. herbarum, Parathyridaria flabelliae, and Lophiostoma sp. moulds. Most of these fungi are endophytic or can grow saprotrophic on woody substrate, but also cause infections in plants, creating phytosanitary problems to numerous herbaceous and woody plant species.