Introduction. Efficacy of antibiotics are confirmed by clinical results, however irrational use: exaggerated doses, the use of last resort antibiotics, the patient’s non-compliance with the treatment plan, the interruption of antibiotic therapy, the lack of need for the use of antibiotic therapy etc. results in the development of antibiotic resistance. Problem whose severity progresses with time, a fact also encountered in the urological clinic. Objective. Retrospective data analysis of microbial antibiotic resistance and the usefulness of the measures undertaken to combat its progression in Municipal Clinic Hospital (MCH) ”Sfânta Treime”, Urology Department, 2018-2022 period. Material and methods. The statistical data for the period 2018-2022 from bacteriological laboratory of MCH ” Sfânta Treime” were analyzed. Data were processed using Microsoft Excel and Statistica 7. Results. The most common bacteria met in the urology department were Klebsiela pneumoniae24 %, Enterococcus faecalis-32%, E.Coli 28%, Pseudomonas aeruginosa 6%, other 10%. During 2018-2019 a progressive development of antibiotic resistance was observed in the isolated bacteria mentioned above. The data for 2020 are only available for the first semester, the cause being the SARS-CoV2 pandemic, which made it impossible to properly monitor the hospital microbial spectrum. After Adjusting the antibiotic therapy according to international protocols, the years 2021-2022 don`t show any change in the microbial spectrum nor the level of antibiotic resistance. Conclusion. Compliance with internationally approved treatment schemes has stalled the progression of antibiotic resistance within the Urology section of the MCH ”Sfânta Treime”.