Introduction. Chronic pain is a common problem experienced by stroke patients that can negatively affect their functional outcomes during rehabilitation. This study aimed to investigate the impact of chronic pain on functional outcomes in stroke patients. Methods. We analyzed data from 270 stroke patients who were divided into three pain status groups: none, acute, and chronic. The mean Barthel Index scores were compared among the groups using ANOVA to examine the differences in Barthel Index scores between each group. Results. Data from 270 stroke patients were analyzed, and they were divided into three groups according to their pain status: none, acute, and chronic. The mean Barthel Index scores were 72.7 (SD± 7.8), 70.1 (SD± 9.4), and 57.9 (SD± 12.7) for the none, acute, and chronic pain groups, respectively. The ANOVA scores among the three pain groups showed a significant main effect of pain status on Barthel Index scores, F (2, 267) = 11.218, p < .001. Post-hoc tests revealed that patients with chronic pain had significantly lower Barthel Index scores compared to those without pain. Conclusions. Chronic pain has a significant negative impact on functional outcomes in stroke patients. During rehabilitation should prioritize the management of chronic pain in stroke patients and develop individualized rehabilitation plans that incorporate pain management strategies to optimize functional outcomes in stroke patients experiencing chronic pain.