Business demography allows the performance of businesses in the economy to be measured. Understanding the processes and dynamics underlying the creation, survival, and death of businesses can provide important insight into business behavior, and the mechanisms for generating new jobs and ensuring better economic development at national and regional levels. The analysis of the enterprises’ birth and death events provides the necessary information on the challenges faced by enterprises and implicitly represents a basis for entrepreneurship development. The purpose of the undertaken research is to analyze the available indicators of the enterprises’ demography in the Republic of Moldova for the period 2016-2020 at the national and regional levels. Research techniques are based on secondary data analysis, comparison, grouping, chain indices, dynamic series, and figures. The results indicate a negative dynamic of the population of enterprises in recent years, with an average death rate of enterprises, which exceeds the birth enterprises rate.