DNA is a molecule that holds and transfers genetic information in living organisms. DNA nanotechnology takes this molecule beyond its natural setting and uses its information to create structural patterns and link them together. This area of study has had a significant influence on nanoscience and nanotechnology by providing us with the ability to manipulate molecular self-assembly. In this presentation, we will explore the techniques used to create DNA nanostructures and examine their potential applications, including their use in single-molecule level investigations.