Introduction: Anastomotic colonic leakage represents a life-threatening complication of postoperative period after colorectal surgery. Despite the performances of modern medicine mortality rate remains high, without significant trend of decreasing. Aim of study was to investigate the effect of collagen patch on bursting pressure and adhesion process in experimental colonic anastomosis. Materials and methods: Thirty six rats were divided in two groups: colonic anastomosis was performed and topical collagen patch “Gelaspon” (Ankerpharm GmbH, Germany) was applied in the group I (n=18); colonic anastomosis without collagen patch in the group II (n=18). Results: The average bursting pressure on the 7th POD in the group I was 247.3±3.326 mm Hg vs240.1±2.858 mm Hg in the group II, respectively (NS). The adhesion process in the group I was 1.167±0.307 vs 0.833±0.307 in the group II (p = 0.460). Adhesion process was appreciated according to the score proposed by de van der Hamm. Conclusion: Using of collagen patch for reinforcement of colonic anastomosis does not increase considerably the bursting pressure and adhesion process.