The research was conducted in the Laboratory of Embryology and Biotechnology of the National Botanical Garden (Institute) „Alexandru Ciubotaru”. For the initiation of in vitro culture of cranberry plants – Vacciniumvitis-idaea L. (variety ‘Grintesegen’) and Vaccinium macrocarpon Aiton (variety ‘Piligrim’) the medium was used Woody Plant Medium (after Lloyd and McCown) gelled with powdered agar, the pH of the medium was adjusted to 5.0. Following the researches, the composition of the culture media was established, favorable for the microcloning of the given species. For microcloning cranberry plants the most favorable proved to be nutrient medium Woody Plant Medium gelled with agar and supplemented with zeatin: WPM – 100%, zeatin – 0.5 mg / l, sucrose – 30 g / l, agar – 5 g / l, pH – 5.0. After a period of incubation in the room growth, the emergence and elongation of new shoots were observed in both species studied. However, this medium acted differently on the microcloning of V seedlings macrocarpon AitonandV. vitis-idaea L. Most seedlings of V. macrocarpon Aiton (‚Piligrim’ variety) formed only 3-6 microclones per inoculum, but these microclones were vigorous, well developed, with a height of 8-10 cm, which allows us to get more minibuses from a microclon. Being transferred to the environment rizogenic nutrient, these mini-cuttings had a rooting yield of about 100%. Placement of V.vitis-idaea L. mini-cuttings on the culture medium with zeatin added (Grintesegen variety) favored the induction of lateral buds on the seedling axis, which caused the proliferation of shoots: about 10-12 shoots were formed per inocul. These microclones were fragile, thin (height of 3-5 cm) and being subsequently transferred to rhizogenic nutrient medium, they had a survival and rooting percentage of about 70-80%. The optimal dimensions of the explant for microclonation in both species are 1.5-2 cm.

Cercetările au fost realizate în Laboratorul de Embriologie şi Biotehnologie al Grădinii Botanice Naţionale (Institut) „Alexandru Ciubotaru”. Pentru iniţierea culturii in vitro a plantelor de merişor – Vacciniumvitis-idaea L. (soiul ‚Grintesegen’) şi Vacciniummacrocarpon Aiton (soiul ‚Piligrim’) a fost utilizat mediul Woody Plant Medium (după Lloyd şi McCown) gelificat cu agar pudră, pH–ul mediului a fost ajustat la valoarea 5,0. În urma cercetărilor efectuate a fost stabilită componenţa mediilor de cultură, favorabile pentru microclonarea speciilor date. Pentru microclonarea plantelor de merişor cel mai favorabil s-a dovedit a fi mediu nutritiv Woody Plant Medium gelificat cu agar şi suplinit cu zeatină: WPM – 100%, zeatină – 0,5 mg/l, zaharoză – 30 g/l, agar – 5 g/l, pH – 5,0. După o perioadă de incubare în camera de creştere, s-a observat apariţia şi alungirea noilor lăstari la ambele specii studiate. Totuşi acest mediu a acţionat diferit asupra microclonării plantulelor de V. macrocarpon Aiton şiV. vitis-idaea L. Majoritatea plantulelor de V. macrocarpon Aiton (soiul „Piligrim”) au format doar 3-6 microcloni la un inocul, însă aceşti microcloni erau viguroşi, bine dezvoltaţi, cu înălţimea de 8-10 cm, cea ce ne permite să obţinem mai mulţi minibutaşi dintr-un microclon. Fiind transferaţi pe mediu nutritiv rizogen, aceşti minibutaşi au avut un randament de înrădăcinare de circa 100%. Plasarea pe mediul de cultură adiţionat cu zeatină a minibutaşilor de V. vitis-idaea L. (soiul ‚Grintesegen’) a favorizat inducerea mugurilor laterali pe axul plantulei, cea ce a provocat proliferarea lăstarilor: s-au format circa 10-12 lăstari per inocul. Aceşti microcloni erau firavi, subţiri (înălţimea de 3-5 cm) şi fiind transferaţi ulterior pe mediu nutritiv rizogen, aveau un procent de supraviețuire şi înrădăcinare de circa 70-80%. Dimensiunile optime ale explantului pentru microclonare la ambele specii constituie 1,5-2 cm.