Research objective: to identify tomato genotypes resistant to Alternaria on variability and symptoms of male gametophyte on selective backgrounds with cultural filtrate of pathogens Alternaria spp. A set of gamete breeding techniques and genetic-statistical analysis were used in the experiments. Some patterns of the variability and heritability of traits in the tomato male gametophyte have been identified on media with filtrates of pathogens. The differences in the resistance of pollen to the filtrate influence were established; the differentiation and selection of genotypes for further breeding were made. These studies can be used at different stages of the selection process.