This article presents the results of research, whose purpose was to use alleles AEB-locus of blood groups with individual selection of cattle in animal herds of STE "Maximovca". For individual fixing have been selected 2 bullproducer - Academician 767 and Kiperush 79/395360, which are improvers on a complex of traits with the category А2Б2. Productivity of their mothers was 9331 kg of milk, with fat content 4.90% and 10915 kg of milk with 4,42 percent of fat, respectively. At bull Academician 767 allele of EAB locus - B2O1/G2Y2E'1Q', at bull Kiperush 79 - G2Y2E'1Q'/G". Heifers (mother of the future bulls) belonged to the lines Rozeyf Siteyshna 2671510, Montvik Chifteyna 95679, Pawnee Farm Arlinda Chifa 1427381, of which 47% - marked with allele B2O1, 20,0% - with allele G2Y2E'1Q' 'and 35,4% of the animals are marked with other alleles. Genetic distance between the lines, which include dockable bulls and heifers, is large, thus avoiding a future inbreeding.