There are presented the results of the evaluation of animals of Holsteins of Dutch selection in herd SLL „DokSanCom” for the 2 study periods: Ist (2012, n = 202) and the IInd (2016, n = 144). During the analyzed periods of research in the population of the assessed animals is observed a high frequency of occurrence of antigens B2, G2, I2, O2, Y2, E'2, G″, which is typical for the Holstein breed and other breeds of black motley roots, and also to the Moldovan type of black motley cattle. In the AEC-system is observed an increase in the frequency of occurrence of antigens R1 (0.0247-0.0833), E (0.5742-0.5903), X2 (0.5891-0.6806), L′ (0.0742-0.2083). During the analyzed period of studies were found 21 identical alleles of AEB-locus. The main relative densities in the structure of the allelophond of herd alleles occupy B2O1, B2O1Y2Dʹ, G2Y2Eʹ1Qʹ, I2, D′G′O′, Oʹ, Q′ and G″. Low homozygosity ratio (Cα – 5.0-5.2%) shows the high genetic diversity of the population of studied cattle. In the antigenic spectrum and in the allelophond of AEB locus at cattle of SLL „DokSanCom” predominate marker antigens and alleles characteristic to Holstein cattle, to other breeds of black motley roots, and also to Moldovan type of black motley cattle. Further selection and selection of animals in the herd SLL „DokSanCom” expedient to maintain in view of the genetic blood group systems.