Flavanones are natural compounds commonly occurring in plants either in the free form or as glycosides. They belong to the large group of flavonoids widely distributed in a variety of edible plant sources such as fruits, vegetables, nuts, seeds, grains, tea and wine. Flavanones have health related properties. They show antiproliferative effect and exhibit antiatherogenic and cardiovascular properties. Due to the asymmetric C2 position in their moiety flavanones are chiral compounds and thus can exist in two enantiomeric forms. There are at least two main reasons for which the effective methods of analysis constitute the problem of crucial importance in the flavonoids chemistry. Firstly– because number of their representatives appear in natural mixtures and secondly – because flavonoids’ specific appearance in particular species of plants or fruits make them suitable indicator of quality in food analysis. They may serve for taxonomic purposes and sometimes may supply proofs of adulteration. The chiral separations of flavanones and flavanone glycosides in HPLC system have been elaborated using celulose triacetate and β-cyclodextrin (Cyclobond I) as chiral stationary phases [1, 2]. The current communication reports the applications of amylose and cyclodextrin based stationary phases for enantioseparation of selected flavanones in HPLC conditions. The influence of various mobile phase on enantiorecognition process will be discussed.